ValitaTiter (i3x i3) ValitaTiter (iD5) ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. One sensor measures the temperature of the top chamber heating foil, and another is used to measure the bottom chamber heating foil. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. Quantiferon-TB Gold in Tube Kit. Multimode Readers Comparison Chart. . Simplified workflow: Add cellular imaging assays in a small footprint. . . Background noise is reduced by the reader’s cooled PMT, enabling increased sensitivity and dynamic range. gen) on a Spectramax i3X luminometer. 012 fmol 0. Also refer to this Multimode Reader Comparison Chart. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic G q -coupled receptor. 1 software Download page. 2in. Settings not required by a reader are indicated by ‘---’. Introduction. Eliminate time-dependent substrate addition steps. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. . 302 low ref light. Keywords: htrf spectramax detection cartridge, spectramax i3, spectramax paradigm system, htrf reagents, spectramax i3 and paradigm readers, HTRF assay, htrf. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Find regional support. Explore PDF for more details. The spectra analysis and K d were determined using Sigmaplot 14. The SpectraMax i3x reader provides the The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Prepare your i3/i3x instrument for shipment. The SpectraMax Paradigm Multi-Mode Detection Platform is a modular multi-mode microplate detector. i3x Multi-Mode Microplate Reader. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. In this product video, see how the SpectraMax M5 quickly reads samples. GloMax Discover System. The dual auto injector module. Analysis. ai; ImageXpress Pico; IN Carta Image Analysis Software; CLONE SCREENING. 314 low lamp level warning. The SoftMax Pro Software provides integrated instrument control. ZERO BIAS - scores, article reviews, protocol conditions and moreStructured Review. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. The user guide also covers the features and specifications of the SpectraMax MiniMax 300. Antigen Quantitation Protocol. . For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part. EUROPE. For more sample throughput, the system canMicroplates were sealed to reduce evaporation (adhesive PCR plate seal; Thermo Fisher Scientific, AB0558) and placed in a microplate reader (Molecular Devices, SpectraMax i3x) for 8 h at 30 °C. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. 2 only) SpectraMax M-series Microplate readers. Download PDF. We would like to show you a description here but the site won’t allow us. BMG LABTECH VANTAstar. 3 Figures from 612 Publications Promega. 58% for the detection module and 2. 더 많은. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Capture Flash Assays with Ease. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. *On the SpectraMax i3x reader only. were performed on a Biomek i7 containing an integrated orbital plate shaker and a SpectraMax i3x plate reader (Molecular Devices). They include the SpectraMax® iD3 Multi-Mode Microplate Reader, SpectraMax® L Microplate Reader, SpectraMax® i3x Multi-Mode Microplate Reader, and SpectraMax® M5 Multi-Mode Microplate Reader. Winnersh Triangle. TP medium was used as a blank and its fluorescence intensity was subtracted from that of mCherry or from chlorophyll auto-fluorescence intensity. Downloads. 0´ 104 1. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. HTRF-compatible SpectraMax Multi-Mode Readers. SpectraMax Quant dsDNA Assay Kits. Figure 4. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Sensitive TRF detection of two simple, mix-and-measure assay workflows. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. . infoboxeu@moldev. pH-Xtra Glycolysis Assay - Agilent. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. SpectraMax i3x; SpectraMax iD3 / iD5; SpectraMax M Series; SoftMax Pro Software; CELLULAR IMAGING. Description. GM3000 Register or Sign In. Find our technical support information along with contact details. 313 reference gain check fail. SpectraMaxParadigmMulti-ModeDetectionPlatformUserGuide 4 5014038F Chapter5:ReadModesandReadTypes 71 SupportedReadTypes 71 AbsorbanceReadMode 74 FluorescenceIntensityReadMode 79the SpectraMax Paradigm Multi-Mode Detection Platform User Guide. i3x. SoftMax Pro. GFP expression was higher at 21 hours post transfection than at 16 hours (16-hour data not shown). This update is applicable for users of the SpectraMax® i3x Multi-Mode Detection Platform and SpectraMax® Paradigm® Multi-Mode Microplate Reader with the ScanLater Western Blot (WB) Detection Cartridge and the SpectraMax® iD5 Multi-Mode Microplate Reader: SMP 7. Unpacking and setting up the instrument includes the following procedures: • Unpacking the instrument and saving the original packaging. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Molecular Devices. Data analysis. Expanded dynamic range The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube. Spectranet customer portalThe SpectraMax i3x measures monochromator-based absorbance, fluorescence and luminescence with a future-ready platform that can evolve with the researcher’s needs. Unlimited breadth of. Filterless readers provide consistent results with steady temperature regulation. Shaking and incubations were performed using an on-deck orbital shaker. using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. SpectraMax i3/i3x Multi-Mode Microplate Reader SoftMax Pro Software 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg/we l) R F U 02 4 6 8 1 0 2. Page 9 Setting Up the SpectraMax® MiniMax™ 300 Imaging Cytometer 2. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. to View Figures. Open the catalog to page 1. (SpectraMax i3/i3x) CatchPoint SimpleStep ELISA (SpectraMax Paradigm) SpectraDrop. For each kit, the reader was able to detect the lowest dilution as positive. We would like to show you a description here but the site won’t allow us. 13 There are a fairly limited number ofSARS-CoV-2 variants of concern (VOCs) that increase transmission or disease severity or reduce diagnostic or vaccine efficacy continue to emerge across the world. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. . Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. We would like to show you a description here but the site won’t allow us. For each assay kit, a preconfigured protocol is included in SoftMax Pro Software to facilitate the acquisition and analysis of data for a simplified workflow. The calculated LOQ values were 0. 3. The plate was shaken and incubated in the dark for 10 minutes. 5 decades, with sensitive detection of cells. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Measured using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. STAKMAX. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. SpectraMax i3x Multi-Mode Microplate Reader. Contact Local Team. We would like to show you a description here but the site won’t allow us. ISM Equipment Classification (Group 1, Class A) This equipment is. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. Promega. 99 to $1999. It allows you to explore cellular . The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. The SpectraMax i3x reader allows one to unravel the mysteries of science by exploring cellular pathways and protein. 在这里，我们展示了如何使用SpectraMax®i3x和 SpectraMax®iD5多功能微孔板读板机应用HTRF技术进行 Gq-偶联受体的分析。G蛋白偶联受体的分析主要通过检测 cAMP和IP3这两条信号转导通路调控介导的细胞内钙离子水 平的升高。Cisbio公司的IP-One Gq试剂盒通过检. Find our technical support information along with contact details. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. View the Molecular Devices website. com The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. spectramax i3x - by Bioz Stars , 2023-11. . Ease of use Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析Spectramax i3x fluorescence plate reader to measure fluo-rescence resonance energy transfer (FRET) signals from cell lines expressing CFP-YFP FRET sensors for glucose and ATP, as these sensors give large changes in FRET ratio in response to appropriate conditions, and are expressed in cells at high levels. Explore a wealth of applications with the future-ready SpectraMax i3x. Multimode Reader Comparison Chart. The SpectraMax i3x’s user-upgradeable application modules will future-proof your research to include most assay capabilities of the SpectraMax iD3 and iD5 readers, as well as additional capabilities such as imaging. 1 Microplate Data Acquisition & Analysis Software for Windows. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. . 2. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. . 2 or higher,. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Published Dec 16, 2020 | Updated Oct 10, 2023. The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely. . Find regional support. 7 mm for all shake intensities. Adding time resolved fluorescence, HTRF® or western blot detection simply requires the user to exchange a detection module. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. Figure 3. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. Unlimited breadth of. The SoftMax® Touch Software is installed in. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. The corrected absorbance is within 5% of the. Find regional support. Instrument. . 306 plate air cal fail,low light. 030 fmol. Sensitive TRF detection of two simple, mix-and-measure assay workflows. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex. Eight different automated tests are provided: • Absorbance accuracy (linearity) • Precision (reproducibility) • Stray light • Wavelength accuracy • Wavelength repeatability SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Cet appareil ISM est confomre à la norme NMB-001 du Canada. ImageXpress Confocal HT. The information below applies to a standard 96 well plate, set to a linear shake mode. Multimode Reader Comparison Chart. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThis characterization of nanoparticle and its surface interactions can be detected using the SpectraMax i3x Multi-Mode Microplate Reader and the Spectral Optimization Wizard in SoftMax Pro Software. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. Video: SpectraMax i3x Overview; Brochure: SpectraMax i3x Multi-Mode Detection PlatformVersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. 吸収度マイクロプレートリーダー SpectraMax® Mini. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. Products : SpectraMax i3x reader, SoftMax Pro GxP Software, SpectraMax i3, SoftMax Pro Software, SpectraMax MiniMax cytometer When I attempt a read with my SpectraMax i3 or i3x reader using the injector cartridge, why do I see a plate height error, even though I have entered the correct plate height?. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. ATP LLD (pM) ATP LLD (moles) SpectraMax i3x, luminescence . Description 1. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesWith these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. The information is below the horizontal line. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. 6% for the on-board optics (monochromators). Wait 20 seconds. The SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer is equipped with transmitted light (TL), green fluorescence (Ex/Em: 460/541), and red (625/713) fluorescence channels. Figure 1. Unparalleled performance on a personalized platform. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 7 Figures from 1126 Publications Promega. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. Results were plotted as ratio of green/red vs. SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. 98 / 100 stars. SpectraMax i3x Multi-Mode Detection Platform. The calculated LOQ values were 0. Monochromator optics with adjustable bandwidth offer excellent sensitivity down to 6 nM tryptophan, as well as precise determination of the fluorescence emission peak shift that occurs upon protein denaturation. Italic and boldface type are used for emphasis. Absorbance Fluorescence (top/bottom read) Luminescence (top read) Time-Resolved Fluorescence (top read). . SpectraMax® i3x. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. StackMax Microplate Handling System. 001 0. Thetypeof luciferase(Renilla. Summary of Contents for Molecular Devices SpectraMax M2. Unplug the power cable from the back of the instrument. The SpectraMax DuoLuc Reporter Assay has been performance optimized on the SpectraMax i3x reader with Injector Cartridge. Biochrom New EX Read 400 Microplate Reader. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. 1321N1, a human astrocytoma cell line. 99 to $1999. . Price from $9. The CellTiter-Glo assay from Promega uses luciferase. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. 3: The cuvette chamber. Subsequently, 100 μL of the substrate solution (2 mM of p-nitrophenyl butyrate in dimethylformamide) was added into the mixture, and incubated at 37°C for 15 min. SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. 305 reference level saturation. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. As an aspirant Bioscience research with expertise in cell biology, immunology and bio instrumentation techniques, I bring a wealth of knowledge and expertise to the table. As per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideProducts : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. R8362. Homogeneous and eterogeneous microplate assays can be performed in one flexible system. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Reader spectramax microplate id3 molecular devices multi mode boundaries functionality instrumentSpectramax i3x multi-mode microplate reader with minimax imaging Molecular devices spectramax i3x 使用される 販売のために 価格. 因为是导师第一任学生，没有师兄师姐，加上本人悟性也差，小小pcr花了半年时间才终于有点样子。因为经历过，所以懂得，故而分享之，希望能给到屏幕前的你一点点帮助! 如有失误，欢迎指正~最后，不要忘了一键三联哟，谢谢大家！, 视频播放量 6293、弹幕量 2、点赞数 122、投硬币枚数 55、收藏. . Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. The ELISA plates were read at an absorbance of 450 nm on a SpectraMax i3x Plate Reader using Softmax Pro 7. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. 9000-0756. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. SeeThe SpectraMax i3x measures monochromator-based absorbance, fluorescence and luminescence with a future-ready platform that can evolve with the researcher’s needs. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. Order Now. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when. The SpectraMax i3x reader grows with you as you continue to advance your research, enabling you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. to View Figures. SpectraMax i3x; SpectraMax iD3 / iD5; SpectraMax M Series; SoftMax Pro Software; CELLULAR IMAGING. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. . The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring Download SoftMax® Pro 7. 0001 0. D 1 1. Molecular Devices are known for both the reliability and the wide uses of their products. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. SoftMax Pro. SpectraDrop Abs Protein Quant (i3-i3x)Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Figure 4. Determination of anti-inflammatory. These robust readers have been placed in labs from Antarctica to the. 2 nM was calculated using SoftMax Pro Software. Cell counters for accurate and. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Adding time resolved fluorescence, HTRF® or western blot detection simply requires the user to exchange a detection module. com. EMax Plus Microplate Reader. . It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. 価格、見積もり、お近くの販売店を. (3) Molecular Devices, SpectraMax i3x, Microplate Readers (2) 2022 (1) 2020 Invetech Korus KRS-100 Elutriation & Washing Systems (3) 2021 ThermoFisher Scientific, invitrogen, CytKick Max AutoSampler w/ Attune NxT: Acea NovoCyte Flow Cytometer: Appliedbiosystems, QuantStudio 7 Flex, Real-Time PCR SystemThe SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. . Then, cells were washed with PBS three times and 100 μL of Nano-Glo HiBiT Lytic Reagent was added to. 003 fmol 0. o For example: Ex: 485/Em 525/Cutoff 515 4. Pancreatic lipase activity was determined by measuring the absorbance of the test solution at 405 nm with a micro plate reader (MD SpectraMax i3x). The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyMeasure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. Tristar 5 Multimode Microplate Reader. Register to download your free eBook today. Description 1. SpectraMax Microplate Readers. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellEvery SpectraMax ® Paradigm Detection Cartridge starts with an application optimized excitation energy source either in the form of a long life, high powered LED, xenon flash lamp, or high powered laser. Amplitude : 1. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. Download PDF. Recommended settings for SpectraMax iD5 and i3x readers. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular. However, no piece of equipment is immune to breaking down. which chemiluminescence was detected on a SpectraMax i3x reader (Molecular Devices) and analyzed us ing GraphPad. SpectraMax i3x readers. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. The calculated LOQ values were 0. Figure 2-1 Label on Rear of Instrument WARNING!We would like to show you a description here but the site won’t allow us. Tip: MCF-7 cells have a polygonal morphology with irregular dimensions, and they have a habit of clumping into large aggregates. SpectraMax iD3 and iD5 Register or Sign In. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyThe SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. All test measurements and calculations are handledPreconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. ai; ImageXpress Pico; IN Carta Image Analysis Software; CLONE SCREENING. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. The SpectraMax® DuoLuc™ Reporter Assay Kit enables highly sensitive quantitation of both firefly and Renilla luciferases in mammalian cells at a great price. Product Details. In order to calculate a humification index (HIX), spectra were obtained with an excitation wavelength of 254 nm over the emissions waveband of 300–480 nm at increments of 5 nm (Ohno 2002. 7. Modularity does provide some measure of forward vision into upcoming or future experimental needs. Genscript cPass SARS-CoV-2 Neutralization Antibody Detection. Time-. ü; SpectraMax® Mini reader support. . 0. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. SpectraMax Paradigm Platform in conjunction with AlphaLISA provides a powerful tool to screen novel therapeutics in a pathologically relevant, endothelial cell-based model. The SpectraMax i3x reader provides theThe SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. 2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. 99. i3x single use: $35: $52. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. Learn more about its features, specifications, and applications in this user guide. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. . Spectranet customer portal Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. 99. SpectraMax i3x Register or Sign In. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Designed specifically for the SpectraMax® i3 Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick inspection of cells and provides insight into phenotypic changes that are important for understanding cytotoxicity, cell proliferation, and protein expression. Research Article AntioxidantActivity,PolyphenolicContent,andFT-NIR AnalysisofDifferentAspiliaafricanaMedicinalPlantTissues DenisOkello ,1,2,3YuseongChung,1HyoseonKim. Molecular Devices ScanLater Western Blot Detection Platform. . The SpectraMax i3x reader allows you to unravel the mysteries of science by exploringLuciferase activity was measured using a SpectraMax i3x microplate reader (Molecular Devices). Prepare your i3/i3x instrument for shipment. +44-118-944-8000. A 1:2 dilution series of BSA was prepared in PBS, starting at 100 mg/mL, in LoBind microcentrifuge tubes. Superior flexibility The SpectraMax i3 Multi-Mode Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. SoftMax Pro User’s Manual v Contents Figures. It allows you to explore cellular pathways and protein activation and expression in one system. Curves were forced through x,y = 0. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). 306 plate air cal. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. 305 reference level saturation. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Our absorbance plate readers feature our PathCheck Sensor. Bioz Stars score: 98/100, based on 1 PubMed citations. Detection cartridge modularity allows for easy addition of new read modes within minutes without. 0´ 104 4. Spectranet customer portalThe SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. Figure 2-23: Bottle holder To install the bottle holder: 1. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. The 4x washing steps were also performed on deck using the Span-8 aspirate and dispense for the first three washes. Bioz Stars score: 98/100, based on 1 PubMed citations. Average 98 stars, based on 1 article reviews. SpectraMax i3/i3x readers must be equipped with a suitableThe SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. . SpectraMax i3x Multi-Mode Detection Platform. This process should take about 3 - 5 minutes. My proficiency in techniques such as •PBMC isolation, counting and. Cell viability는 세포막 Integrity 또는. SpectraMax® i3x Multi-Mode Detection Platform; SpectraMax® i3 Multi-Mode Detection Platform; SpectraMax® ID3 Multimode Microplate reader; SpectraMax® ID5 Multimode Microplate reader; SpectraMax® Paradigm® Multi-Mode Microplate Reader; SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. Spectranet customer portal(SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published microplate reader control and microplate data analysis software package available to scientists. VAL014, right table) assays on the SpectraMax i3x reader are shown below. . . SpectraMax iD5:SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. StakMax Handling Stacker Base Unit. Failed to load PDF file. Not compatible with Home Edition. 303 unable to cal dark current. Automated for ease of use. 세포신호전달은 세포가 환경에 반응하고 다른 세포와 소통할 수 있게 합니다…. Read more about HTRF SpectraMax Detection Cartridges for SpectraMax i3x & Paradigm Systems which utilize a high energy Xenon flash lamp for the sensitive reading of HTRF reagents. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT.